Open AccessRac1b recruits Dishevelled and β-catenin to Wnt target gene promoters independent of Wnt3A stimulation
Author(s) -
Vaijayanti Pethe,
George S. Charames,
Bharati Bapat
Publication year - 2011
Publication title -
international journal of oncology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.405
H-Index - 122
ISSN - 1019-6439
DOI - 10.3892/ijo.2011.1066
Subject(s) - wnt signaling pathway , promoter , biology , chromatin immunoprecipitation , dishevelled , carcinogenesis , beta catenin , microbiology and biotechnology , chromatin , activator (genetics) , catenin , cancer research , gene , signal transduction , genetics , frizzled , gene expression
We previously reported a functional interaction between aberrant Wnt signaling and Rac1/Rac1b GTPases in tumorigenesis. In this study, we further investigated the mechanistic role of nuclear Rac1b. Using chromatin immunoprecipitation (ChIP) studies, we show that Rac1b resides at the promoters of Wnt target genes, c-Myc and Cyclin D1, in HCT116 cells with aberrant Wnt pathway. In HEK293T cells with intact Wnt signaling, Rac1b is tethered to these same gene promoters independent of Wnt3A stimulation and is further observed to recruit Dishevelled and β-catenin in the absence of Wnt3A stimulation. Our studies suggest a novel transcriptional co-activator role of Rac1b in β-catenin/TCF-mediated transcription.