Open Access1α,25-Dihydroxyvitamin D3 inhibits the differentiation and bone resorption by osteoclasts generated from Wistar rat bone marrow-derived macrophages
Author(s) -
Dong Wang,
Jianhong Gu,
Chen Yang,
Hongyan Zhao,
Wei Liu,
Ruilong Song,
Jianchun Bian,
Zongping Liu,
Yan Yuan,
Zongping Liu
Publication year - 2015
Publication title -
experimental and therapeutic medicine
Language(s) - English
Resource type - Journals
eISSN - 1792-1015
pISSN - 1792-0981
DOI - 10.3892/etm.2015.2632
Subject(s) - bone resorption , cathepsin k , osteoclast , endocrinology , chemistry , medicine , rankl , bone remodeling , bone marrow , acid phosphatase , tartrate resistant acid phosphatase , microbiology and biotechnology , biology , receptor , biochemistry , activator (genetics) , enzyme
The steroid hormone 1α,25-dihydroxyvitamin D 3 [1α,25-(OH) 2 D 3 ] plays an important role in maintaining a balance in calcium and bone metabolism. To study the effects of 1α,25-(OH) 2 D 3 on osteoclast (OC) formation and bone resorption, OC differentiation was induced in bone marrow-derived mononuclear cells from Wistar rats with the addition of macrophage colony stimulating factor and receptor activator for nuclear factor-κB ligand in vitro . Cells were then treated with 1α,25-(OH) 2 D 3 at 10 -9 , 10 -8 or 10 -7 mol/l. OCs were identified using tartrate-resistant acid phosphatase staining and activity was monitored in the absorption lacunae by scanning electron microscopy. Expression levels of functional proteins associated with bone absorption, namely carbonic anhydrase II, cathepsin K and matrix metalloproteinase-9 were evaluated by western blot analysis. The results showed that 1α,25-(OH) 2 D 3 inhibited the formation and activation of OCs in a dose-dependent manner and downregulated the expression levels of bone absorption-associated proteins.