Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells | Zendy

ChunPeng Zhao | Zendy; Zhong-Jie Xu | Zendy; Qing Guo | Zendy; Yunxiao Li | Zendy; Xiang-Zheng Gao | Zendy; Yi-You Peng | Zendy

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Overexpression of suppressor of IKBKE 1 is associated with vincristine resistance in colon cancer cells

Author(s) -

ChunPeng Zhao,

Zhong-Jie Xu,

Qing Guo,

Yunxiao Li,

Xiang-Zheng Gao,

Yi-You Peng

Publication year - 2016

Publication title -

biomedical reports

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.607

H-Index - 25

eISSN - 2049-9442

pISSN - 2049-9434

DOI - 10.3892/br.2016.759

Subject(s) - oncogene , cancer research , biology , colorectal cancer , cancer , microbiology and biotechnology , cell cycle , genetics

In a previous study, the suppressor of IKBKE 1 expression level was confirmed to be higher in vincristine (VCR)-resistant HCT-8 (HCT-8/V) colon cancer cells than in non-VCR-resistant HCT-8 cells. In the current study, IKBKE 1 expression in VCR-resistant colon cancer cells was investigated further. HCT-8 and HCT-8/V human colon cancer cells were used, and polymerase chain reaction (PCR) primers were designed to amplify the IKBKE 1 gene. Fluorescence reverse transcription-quantitative PCR (RT-qPCR) was performed to detect differences in IKBKE 1 expression between sensitive and drug-resistant colon cancer cell lines. Western blotting was performed to further observe IKBKE 1 expression. Based on the RT-qPCR and western blot results, IKBKE 1 expression was observed to be markedly higher in the HCT-8/V cells, and this difference was significant (P<0.05). Thus, IKBKE 1 expression was identified to be associated with the resistance of colon cancer cells to VCR.

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