Open AccessTricistronic hepatitis C virus subgenomic replicon expressing double transgenes
Author(s) -
Xin Cheng
Publication year - 2014
Publication title -
world journal of gastroenterology
Language(s) - Uncategorized
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v20.i48.18284
Subject(s) - replicon , internal ribosome entry site , virology , luciferase , biology , microbiology and biotechnology , transfection , rna , hepatitis c virus , plasmid , virus , gene , ribosome , genetics
To construct a tricistronic hepatitis C virus (HCV) replicon with double internal ribosome entry sites (IRESes) of only 22 nucleotides for each, substituting the encephalomyocarditis virus (EMCV) IRESes, which are most often used as the translation initiation element to form HCV replicons.