Open AccessConstruction of targeted plasmid vector pcDNA3.1-Egr.1p-p16 and its expression in pancreatic cancer JF305 cells induced by radiationin vitro
Author(s) -
Hongbing Ma,
Xijing Wang,
Zheng-Li Di,
Hui Xia,
Li Zheng,
Jie Liu,
Jie Ma,
Hua-Fen Kang,
Chunyan Wu,
Man Bai
Publication year - 2007
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v13.i31.4214
Subject(s) - transfection , clonogenic assay , microbiology and biotechnology , lipofectamine , biology , trypan blue , flow cytometry , apoptosis , plasmid , viability assay , cell culture , recombinant dna , cancer research , gene , vector (molecular biology) , biochemistry , genetics
To construct pcDNA3.1-Egr.1p-p16 recombinant plasmid and investigate the expression of p16 in pancreatic cancer JF305 cells induced by radiation and the feasibility of gene radiotherapy for pancreatic carcinoma.