Open AccessExpression of survivin in primary and metastatic gastric cancer cells obtained by laser capture microdissection
Author(s) -
Zhenning Wang,
Hui Xu,
Li Jiang,
Xin Zhou,
Chong Lu,
Xue Zhang
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i21.3094
Subject(s) - survivin , laser capture microdissection , microdissection , cancer , lymph node , cancer cell , cancer research , medicine , cell , lymph , pathology , gene expression , biology , gene , biochemistry , genetics
Survivin, a recently identified member of the inhibitor of apoptosis protein family, is expressed during development and in various human cancers. However, its expression in normal tissues and clinical relevance in cancers are still debated. In the present study, we analyzed the expression of the survivin gene in human primary and metastatic gastric cancer cells as well as in paired epithelial cells from normal gastric mucosa by means of a novel laser capture microdissection (LCM) technique coupled with reverse transcription-polymerase chain reaction (RT-PCR).