Open AccessA rapid and efficient method to express target genes in mammalian cells by baculovirus
Author(s) -
Tong Cheng
Publication year - 2004
Publication title -
world journal of gastroenterology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.427
H-Index - 155
eISSN - 2219-2840
pISSN - 1007-9327
DOI - 10.3748/wjg.v10.i11.1612
Subject(s) - sf9 , lipofectamine , transduction (biophysics) , biology , cell culture , recombinant dna , reporter gene , retrovirus , microbiology and biotechnology , viral vector , transfection , virology , recombinant virus , hek 293 cells , baculoviridae , flow cytometry , virus , multiplicity of infection , gene expression , spodoptera , gene , vector (molecular biology) , genetics , biochemistry
To investigate the modification of baculovirus vector and the feasibility of delivering exogenous genes into mammalian cells with the culture supernatant of Spodoptera frugiperta (Sf9) cells infected by recombinant baculoviruses.