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Low‐copy nuclear primers and ycf1 primers in Cactaceae
Author(s) -
Franck Alan R.,
Cochrane Bruce J.,
Garey James R.
Publication year - 2012
Publication title -
american journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.218
H-Index - 151
eISSN - 1537-2197
pISSN - 0002-9122
DOI - 10.3732/ajb.1200128
Subject(s) - biology , intron , intergenic region , phylogenetic tree , plastid , nuclear gene , gene , genetics , evolutionary biology , genome , chloroplast
• Premise of the study: To increase the number of variable regions available for phylogenetic study in the Cactaceae, primers were developed for a portion of the plastid ycf1 gene and intron‐spanning regions of two low‐copy nuclear genes ( isi1 , nhx1 ). • Methods and Results: Primers were tested on several families within Caryophyllales, focusing on the Cactaceae. Gel electrophoresis indicated positive amplification in most samples. Sequences of these three regions ( isi1 , nhx1 , ycf1 ) from Harrisia exhibited variation similar to or greater than two plastid regions ( atpB‐rbcL intergenic spacer and rpl16 intron). • Conclusions: The isi , nhx , and ycf1 primers amplify phylogenetically useful information applicable to the Cactaceae and other families in the Caryophyllales.