Open AccessEXPERIMENTAL JUSTIFICATION OF THE EFFICIENCY OF BIOLIDE DISINFECTANT FOR DISPOSAL OF BACTERIAL INFECTIONS IN CONDITIONS OF INDUSTRIAL POULTRY
Author(s) -
O. M. Chechet,
В. Л. Коваленко,
Т. О. Гаркавенко,
O. I. Gorbatyuk,
Tamara Kozytska,
V. O. Andriyashchuk
Publication year - 2021
Publication title -
naukovo-tehnìčnij bûletenʹ deržavnogo naukovo-doslìdnogo kontrolʹnogo ìnstitutu veterinarnih preparatìv ta kormovih dobavok ì ìnstitutu bìologìï tvarin
Language(s) - English
Resource type - Journals
eISSN - 2664-5610
pISSN - 2410-9029
DOI - 10.36359/scivp.2021-22-2.48
Subject(s) - disinfectant , serial dilution , poultry farming , agar dilution method , productivity , microbiology and biotechnology , pulp and paper industry , toxicology , veterinary medicine , biology , antimicrobial , chemistry , medicine , engineering , minimum inhibitory concentration , alternative medicine , organic chemistry , macroeconomics , pathology , economics
The materials of the article are devoted to the study of the bactericidal action of the newly developed «Biolaid» disinfectant at the first stage to determine the possibility of its use in the poultry industry to replace expensive, often toxic and ineffective disinfectants that have now flooded the market in Ukraine.
Modern poultry farms are integrated enterprises, the structure of which consists of one continuous link with an incubator, own parent and industrial herds, production shops for processing products and feed production. One of the most important factors in the successful operation of such enterprises is quality disinfection.
The problem of developing new cheap, safe, effective disinfectants remains relevant. The problem is supplemented by questions on the study of the effects of disinfectants on the indicators of general resistance in poultry and their impact on the productivity of birds, as such information is quite limited.
Bacterial suspensions of test cultures with a concentration of 0.5–1.0 according to the McFarland standard (microbial load from 1.35×108 to 3.0×108 CFU/cm3) were prepared for the study by washing the colonies of the daily culture of microorganisms with trypton-soy agar (TCA) with sterile saline. The working dilutions of the experimental disinfectant were made according to the recommendations in the leaflet-tab, in concentrations of 0.1; 0.2; 0.25 and 0.5 % at exposures of 10, 20 and 30 minutes.
The results of studies of the newly developed «Biolaid» disinfectant showed its high efficiency, proven experimentally, because at the level of 0.25 % concentration of the working dilution of the drug at an exposure of 30 minutes. complete neutralization of test cultures of Staphylococcus aureus ATCC 6538 and Pseudomonas aeruginosa ATCC 15442 was achieved without bacteriostatic effect, which testified to the effectiveness of the drug in its action on gram-negative and gram-positive pathogenic bacteria.