STUDY OF BIOLOGICAL EFFICIENCY AND ECOLOGICAL TOXICITY OF BAIT FOR DESTRUCTION OF RATS AND MICE «BIOSAN» ON LABORATORY RODENTS

The results of research show that the bait for the destruction of rats and mice «Biosan» rats eat willingly, completely. At simultaneous setting, in portable feeders, standard granulated feed and feed substrates of the manufacturer, rodents prefer the latter. On the 2nd day in experimental rats there was a slight inhibition of behavioral reactions, frequent approaches to drinking. There was a slight increase in the frequency of respiratory movements – by 10 units on average. No clinical changes were detected in experimental mice. On day 3 of the study, the average respiratory rate increased to 155 units per minute. In animals, there was a tendency to increase heart rate - tachycardia by 8,95 %. In two cages with rats - refusal of food, in others - reduction of food intake by 35 %. In one specimen, the rat has a visible leak from the nostril, the hair is bulging, there are traces of subcutaneous bleeding around the anus, there is no reaction to touch. No clinical changes were detected in the experimental mice, the food was eaten. Blood tests showed anemia, leukopenia, thrombocytopenic purpura, characteristic of inflammatory processes in the respiratory tract and gastrointestinal tract. Interstitial pneumonia, rupture of the lateral venules of the left thigh, and cardiopathy were found in rats euthanized. On the fifth day, all experimental rats died. Experimental mice showed signs of anemia (mucosal cyanosis) and behavioral disturbances, loss of appetite, or refusal to feed. Experimental mice died for 7-8 days. The carcasses of animals are bluish, swollen, with signs of cardiopathy and pathology of the gastrointestinal tract. The results of histological examinations and morphometric parameters in organs and tissues indicate that the initial changes occur in the immune organs (lymph nodes and spleen), and then the pathological process involves the lungs, heart, kidneys, liver and other organs. Bait for the destruction of rats and mice «Biosan» is made from concentrate «Bromadialone, 1,0%, solution», which, according to the parameters of acute oral and dermal toxicity, belongs to the 2nd class of highly dangerous compounds in relation to DSanPiN 8.8.1.2.002 -98 and SOU 85.2-37-736: 2011; does not pose an inhalation hazard (the active substance is not a volatile substance), but has an extremely pronounced cumulative property. In the future it is planned to carry out complex deratization with the use of the drug «Bait for the destruction of rats and mice «Biosan».