Open AccessCOMPARATIVE ACTIVITY OF INFLUENZA VACCINES: EFFECT ON LYMPHOCYTE SUBPOPULATION STRUCTURE
Author(s) -
E. A. Chromova,
L. A. Semochkin,
Elina Akhmatova,
В. Н. Столпникова,
С. А. Сходова,
Е. В. Сорокина,
Н. К. Ахматова,
М. П. Костинов
Publication year - 2016
Publication title -
žurnal mikrobiologii, èpidemiologii i immunobiologii
Language(s) - English
Resource type - Journals
eISSN - 2686-7613
pISSN - 0372-9311
DOI - 10.36233/0372-9311-2016-6-61-65
Subject(s) - immune system , il 2 receptor , immunology , foxp3 , flow cytometry , biology , peripheral blood mononuclear cell , influenza vaccine , ctl* , virology , in vitro , t cell , vaccination , cd8 , biochemistry
Aim. Study subpopulation structure of lymphocytes in healthy individuals under the effect of various influenza vaccines in an in vitro system. Materials and methods. Evaluation of immunephenotype features of PBMCs, activated in vitro by immune-adjuvanted and unadjuvanted vaccines against influenza in healthy individuals, was carried out by using flow cytometry method. Results. Grippol plus vaccine caused a more pronounced stimulating effect compared with subunit and split-vaccines on NK-cells, cells with markers of early activation CD45/CD25, induced the quantity of natural regulatory cells (CD4/CD25/Foxp3), increase of the number of В-cells and reduced the amount of cell types with apoptosis marker CD45/CD95. Conclusion. Immune-adjuvanted vaccine Grippol plus induced formation of effectors of both innate and adaptive immunity and possessed the most powerful potential regarding activation of various types of immune-competent cells compared with unadjuvanted vaccines.