Open Accessidentification of phylloquinone in cattle samples using T.L.C. method
Author(s) -
L. Bara
Publication year - 2010
Publication title -
acta agraria debreceniensis
Language(s) - English
Resource type - Journals
ISSN - 2416-1640
DOI - 10.34101/actaagrar/i/8379
Subject(s) - chromatography , densitometry , thin layer chromatography , chemistry , chloride , vitamin , methanol , biochemistry , medicine , organic chemistry
A case of hemorrhage of unknown origin was observed in cattle and liver samples were submitted for assay of Vitamin K orpossible cuomarol. After evaluating normal and reverse phase Thin Layer Chromatography (TLC) plates and different solvents,reverse phase TLC plate with indicator and methylene chloride:methanol 70:30 were chosen for direct method of detection andindication of Vitamin K.Gass Chromatography and Densitometry were used to quantitate the Vitamin K present in liver samples andconfirmation of Vitamin K was done by Mass Spectroscopy.