Open AccessANTI-IDIOTYPIC MONOCLONAL ANTIBODIES AGAINST MORPHINE: PRODUCTION, PROPERTIES AND PROSPECTS OF USE
Author(s) -
А. В. Трофимов,
А. Я. Рак,
А. Г. Берзина,
Л. И. Ульянова,
Н. Б. Гамалея,
Т. А. Климова,
Н. В. Станкова
Publication year - 2019
Publication title -
biomedicina
Language(s) - English
Resource type - Journals
eISSN - 2713-0428
pISSN - 2074-5982
DOI - 10.33647/2074-5982-15-2-63-68
Subject(s) - monoclonal antibody , antibody , immunoassay , in vitro , chemistry , monoclonal , microbiology and biotechnology , immunology , biology , biochemistry
This paper describes a technique for producing anti-idiotypic monoclonal antibodies (Ab2) against two morphine derivatives. The specificity of the obtained antibodies was investigated using enzyme immunoassay. The biological activity of the antibodies was studied using an in-vitro model of human glioblastoma T98G cell line prolifer ation.