Open AccessSclerotinia sclerotiorum KK-1 isolate from Belarus: pathogenicity factors and glyphosate sensitivity
Author(s) -
Alena V. Kulik,
Margarita A. Shukshina,
А. Н. Евтушенков
Publication year - 2020
Publication title -
žurnal belorusskogo gosudarstvennogo universiteta. bilologiâ/žurnal belorusskogo gosudarstvennogo universiteta. biologiâ
Language(s) - English
Resource type - Journals
eISSN - 2617-3964
pISSN - 2521-1722
DOI - 10.33581/2521-1722-2020-3-54-69
Subject(s) - sclerotinia sclerotiorum , pectinase , mycelium , cellulase , biology , glyphosate , amylase , botany , sclerotinia , fungus , potato dextrose agar , horticulture , sclerotium , incubation , microbiology and biotechnology , enzyme , agar , biochemistry , bacteria , genetics
In the presence work, mycelial growth, sclerotia formation, polygalacturonase, a-amylase and cellulase activities of a Belarusian fungal isolate Sclerotinia sclerotiorum KK-1 collected from infected сarrot (Daucus carota subsp. sativus) were analyzed. It was established that polygalacturonase was induced by pectin and subject to catabolite repression by glucose. The ability of the isolate to produce oxalic acid, an important factor of pathogenesis, was observed throughout fungus incubation on PDA medium amended with bromophenol blue. Glyphosate (commercial counterpart «Tornado») starting from its level in the medium of 200 mg/L caused a significant inhibition of mycelial growth and sclerotia formation. It was observed that a-amylase and cellulase activities were inhibited by glyphosate unlike polygalacturonase activity.