Open AccessAntifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans
Author(s) -
Rafael Pereira,
Guilherme Mendes Prado,
Raquel Oliveira dos Santos Fontenelle,
Francisco César Barroso Barbosa,
Hélcio Silva dos Santos,
Emmanuel Silva Marinho,
Márcia Machado Marinho,
Selene Maia de Morais
Publication year - 2022
Publication title -
research, society and development
Language(s) - English
Resource type - Journals
ISSN - 2525-3409
DOI - 10.33448/rsd-v11i4.27538
Subject(s) - diallyl trisulfide , fluconazole , candida albicans , diallyl disulfide , chemistry , biofilm , broth microdilution , corpus albicans , minimum inhibitory concentration , antifungal , microbiology and biotechnology , antimicrobial , biochemistry , organic chemistry , biology , bacteria , genetics , apoptosis
The objective of the present study was to determine the antifungal activity, antibiofilm and synergic effect of the compounds diallyl disulfide and diallyl trisulfide in combination with antifungal agents against clinical isolates of Candida albicans, including also a computational study. Antimicrobial sensitivity tests were performed using the broth microdilution method to determine the minimum inhibitory concentration against C. albicans strains and modulatory activity using the checkerboard technique. The biofilm formation was evaluated by biomass quantification using the violet crystal staining method. For the study of molecular docking computer simulations. The constituents showed relevant antifungal activity against strains of C. albicans. In the modulatory activity assay, demonstrated a synergistic interaction with fluconazole and amphotericin B, with an increase in its antifungal action. The diallyl disulfide, diallyl trisulfide and fluconazole ligands formed complexes with ALS3 enzyme. Then, both compounds were considered promising products for the development of new drugs to prevent candidiasis.