Open AccessPENENTUAN KADAR FLAVONOID TOTAL DAN NILAI SPF EKSTRAK ETANOL DAUN BUAS-BUAS (Premna serratifolia L.) ASAL KABUPATEN MELAWI PROVINSI KALIMANTAN BARAT
Author(s) -
Weni Puspita,
Heny Puspasari
Publication year - 2021
Publication title -
jurnal ilmu farmasi dan farmasi klinik/jurnal ilmu farmasi and farmasi klinik
Language(s) - English
Resource type - Journals
eISSN - 2716-3814
pISSN - 1693-7899
DOI - 10.31942/jiffk.v18i01.4896
Subject(s) - flavonoid , chemistry , quercetin , polyphenol , ethanol , traditional medicine , food science , organic chemistry , antioxidant , medicine
Premna serratifolia L. leaves contain flavonoids, saponins, polyphenols, and terpenoids,
potentially act as sunscreens. The purpose of this study was to determine the flavonoid content and
SPF (Sun Protection Factor) ethanol extract of Premna serratifolia L. leaves from Melawi Regency,
West Kalimantan Province using UV-Vis spectrophotometer. The Premna serratifolia L. leaves were
extracted by maceration using 70% ethanol solvent, then the filtrate obtained was concentrated with a
rotary vacuum evaporator to obtain a thick extract. The extract was determined for its flavonoid
content using UV-Vis spectrophotometer at a wavelength of 439 nm, and the SPF value was
determined at a concentration of 25, 50, 75, and 100 ppm with a UV-Vis spectrophotometer at a
wavelength of 290-320 nm. The SPF value was calculated and analyzed descriptively using the
Mansur method. The result showed that the average flavonoid content of ethanol extract of Premna
serratifolia L. leaves was 3.70±0.02% calculated as quercetin. The SPF EEDB values obtained from
variations in concentrations of 25, 50, 75, and 100 ppm respectively were 19.95±0.02; 30.08±0.01;
34.58±0.14; and 38.28 ± 0.12, where the SPF value is included in the ultra protection category.
Key words: Premna serratifolia L. leaves, flavonoid content, SPF, UV-Vis Spectrophotometer