Open AccessBiocontrol of Soil Borne Pathogen of Potato Tuber Caused by Rhizoctonia solani through Biosurfactant based Bacillus strain
Author(s) -
Touseef Hussain,
Abrar Ahmad Khan,
Kun Ma
Publication year - 2021
Publication title -
journal of nepal agricultural research council
Language(s) - English
Resource type - Journals
eISSN - 2392-4543
pISSN - 2392-4535
DOI - 10.3126/jnarc.v7i1.36921
Subject(s) - rhizoctonia solani , bacillus subtilis , surfactin , biology , strain (injury) , 16s ribosomal rna , biological pest control , lipopeptide , rhizoctonia , bacillus (shape) , microbiology and biotechnology , food science , horticulture , bacteria , genetics , anatomy
Black scurf disease is a major problem for cultivation of potato worldwide that is managed by synthetic agrochemicals, that are increasing environmental pollution as well as residue in farm produce also. Therefore, now a day use of bio-agents for managing black scurf is attractive feature and need of the hour. A promising bio surfactant OD02 isolate, was isolated from oil contaminated soil, utilizing its secondary metabolite after preliminary biosurfactant screening tests. Furthermore, this strain exhibited positive test with haemolytic activity, emulsification index, oil spreading test, molecular characterization with 16S rRNA universal primers revealed that the strain belongs to Bacillus group, that was further chemically characterized using TLC, exhibiting reddish pink colour as lipopeptide and demonstrated antagonistic activity against Rhizoctonia solani under in vitro using dual culture (45±0.30%) and at 3% concentration under food poisoning method. Bacillus subtilis HussainT-AMU strain is very effective against phytopathogen R. solani and can be more explored in future diseases management strategies.