Open AccessGHR/HindIII Locus Polymorphisms in Intron-2 GHR Gene of Papua Local Chicken
Author(s) -
Muhammad Affan Mu’in,
Agustinus Gatot Murwanto
Publication year - 2021
Publication title -
jurnal sain peternakan indonesia/jurnal sain peternakan indonesia
Language(s) - English
Resource type - Journals
eISSN - 2528-7109
pISSN - 1978-3000
DOI - 10.31186/jspi.id.16.4.315-321
Subject(s) - hindiii , locus (genetics) , biology , restriction fragment length polymorphism , intron , genetics , growth hormone receptor , single nucleotide polymorphism , allele , genotype , gene , microbiology and biotechnology , growth hormone , endocrinology , restriction enzyme , hormone
This study aimed to detect single nucleotide polymorphisms (SNPs) in intron-2 on growth hormone receptor (GHR) gene in Papua local chickens using the PCR-RFLP method to study its relationship with growth characteristics. Data on the bodyweight of 49 chickens aged 1, 2, 3, and 4 months (22 males, 27 females) and DNA samples were used for this study. The DNA fragment of size 718 bp in intron-2 of the GHR gene from the study chicken was successfully amplified using a pair of specific primers. The PCR-RFLP/HindIII analysis results found this locus's two genotypes (HindIII++ and HindIII--). HindIII+ and HindIII- alleles were 0.02 and 0.98, respectively.