Open AccessPreparation of Local Diagnostic Kit for Salmonella typhimurium
Author(s) -
Zainab Sami Habeeb
Publication year - 2009
Publication title -
the iraqi journal of veterinary medicine/al-maǧallaẗ al-ṭibbiyyaẗ al-bayṭariyyaẗ al-’irāqiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2410-7409
pISSN - 1609-5693
DOI - 10.30539/iraqijvm.v33i1.713
Subject(s) - antiserum , salmonella , antigen , antibody , microbiology and biotechnology , immune system , immune sera , immunization , somatic cell , biology , chemistry , virology , immunology , bacteria , biochemistry , genetics , gene
Some of somatic and flageller antgens from both the human and animal isolates were prepared,and were used to Immunize a group of rabbits by intravenous injections at an upgrading doses.Theresults of this immunization showed the gaining of an excellent hyperimmune serum. With the useof absorptions technique amonospecific antiserum was obtained for the specific injected antigenes, thestandardization of the antibodies showed a highly immune response reading. 1: 2560 , 1: 5120 to H:Iand O: 4.5 Antigens respectively for both antisera.This study showed the report for the 1st time the preparation for diagnostic kit for somatic and flagellarantigens to S. typhimurium comparing this method to Widal test with a good results and Response