Open AccessAXILLARY LYMPH NODE STATUS IN PRIMARY BREAST CARCINOMA
Author(s) -
Farwa Batool Shamsi,
Nadia Naseem,
A. H. Nagi
Publication year - 2015
Publication title -
the professional medical journal/the professional medical journal
Language(s) - English
Resource type - Journals
eISSN - 2071-7733
pISSN - 1024-8919
DOI - 10.29309/tpmj/2015.22.05.1278
Subject(s) - medicine , immunohistochemistry , breast cancer , lymph node , breast carcinoma , axillary lymph nodes , axillary lymph node dissection , pathology , histopathology , metastasis , mastectomy , lymph , oncology , cancer , sentinel lymph node
Objectives: The most important prognostic factor in patients of breast carcinomais axillary lymph node metastasis. Current study was conducted to find the frequency of lymphnode metastasis in hundred cases of primary breast carcinoma and association of lymphnode status with immunohistochemical expression of ER/PR,HER2/neu and MMP-1(matrixmetalloproteinase-1).Design: Descriptive study. Period: Aug 2012 to Jun 2013. Setting: U.H.Slaboratory of Morbid Anatomy and Histopathology Lahore. Materials and methods:Onehundred mastectomy specimens with axillary lymph node dissection were included. Aftergross examination, tissue processing and microtomy the tissue slices of 4 micrometer weretaken on frosted and lysine coated slides. H/E and IHC for ER/PR,HER2/neu and MMP-1 weredone according to protocol. Results: Among 100 breast cancer subjects, 72 were positivefor lymph node metastasis while 28 subjects were negative. A significant association betweenlymph node status and ER IHC expression was noticed. When Chi square test was appliedwith p-value of 0.001 was observed.Also a significant association between lymph node statusand PR IHC expression was noticed. Chi square test was applied and the p-value of 0.004 wasobtained.Association between lymph node status and HER2/neu IHC expression was analysed.Chi square test was applied with a p-value of 0.467 was obtained (not significant).A significantassociation between lymph node status and MMP-1 IHC expression was observed. Chi squaretest was applied anda p-value of 0.004 was obtained. Conclusions: Most of the primary breastcarcinomas were presented with axillary lymph node metastasis (72%).Significant associationswere observed between lymph node status and ER/PR immuonohistochemical expressionshowever the association between HER2/neu IHC and lymph node status was not statisticallysignificant. The immunohistochemical expression of MMP-1 is significantly associated withlymph node status. It shows that it is an important marker for metastatic potential in breastcarcinoma.