BLOOD

All forms of mercury are global pollutants having no environmental limits.Human exposure to mercury occurs basically through food chain due to accumulation oforganic forms of mercury in fish. Objectives: The purpose of the present study was to analyzethe effect of phenyl mercuric acetate on plasma and cytosolic fraction GSH. Study Design:Experimental Study. Setting: Department of Pharmaceutical Chemistry, Faculty of Pharmacy,Gomal University, Dera Ismail Khan. Period: 29 January 2011 to 11 march 2012 .StatisticalAnalysis: One-way ANOVA followed by Dunnet’s HSD test. Results: For the estimation ofthiols Ellman’s method was used and was found statistically significant (p < 0.001) decreasein plasma and cytosolic fraction GSH which was dose and time dependent. The plasma GSHcontents drop in 0 to 120 minutes by various concentrations of phenyl mercuric acetate were64.45%, 59.33%, 50.89%, 41.56%, 33.63% and 32.99% while drop in cytosolic fraction GSHlevel from 0 to 120 minutes by different concentrations of phenyl mercuric acetate (PMA) was53.86%, 48.60%, 45.41%, 36.11%, 29.38% and 27.06%.Conclusions: It is clear that duringorganic mercury toxicity the blood components are also affected which is proved from ourresults. With the increase of time ,the mercury toxicity would be more harmful so detoxificationof organic mercury should be done on emergency bases at the earliest with the help of suitablechelating agents along with antioxidant therapy.