Open AccessInfluence of extraction methods on bioactive compounds from Ngoc Linh ginseng callus
Author(s) -
Lieu My Đong,
Nguyen Thanh Triet,
Thị Hoài Nguyễn,
T.K.T. Dang,
D.G. Do
Publication year - 2021
Publication title -
food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.218
H-Index - 7
ISSN - 2550-2166
DOI - 10.26656/fr.2017.5(3).590
Subject(s) - cellulase , saponin , polyphenol , chemistry , polysaccharide , extraction (chemistry) , ginseng , incubation , amylase , chromatography , food science , enzyme , traditional medicine , biochemistry , medicine , antioxidant , alternative medicine , pathology
Panax vietnamensis Ha et Grushv or Vietnamese ginseng is a valuable medicinal herbwith high economic value in the world. In this study, three target compounds weresurveyed as polyphenols, polysaccharides, and saponins to evaluate the effects of factorsincluding microwave, ultrasound, enzyme amylase, and cellulase for the first time. Theresults of the study showed that with different extraction conditions, the recoveryefficiency of polyphenol, polysaccharide, and saponin was also different. With theaddition of 1.2% of the enzyme amylase (v/v) in 8 hrs of incubation, the highest totalcontent of polyphenol and polysaccharide was obtained, equivalent to 6.48 mg GAE/gsample and 312.48 mg Glu/g sample. While the total saponin content reached the highestvalue of 4.60 mg/g sample at 4 hrs of incubation using 0.8% of cellulase enzyme (v/v).The effect of microwave and ultrasound also showed a significant recovery efficiency forthe three analyzed compounds. But compared to the use of two enzymes (amylase,cellulase), the efficiency was lower. The use of these extraction techniques was based onthe advantages of environmental friendliness, simple operation, lower investment costs,and power saving. At the same time, it can improve the extraction and recovery efficiencyas well as the activity of bioactive compounds.