Open AccessUJI AKTIVITAS ANTIOKSIDAN SENYAWA FLAVONOID PADA KULIT BATANG TUMBUHAN GAYAM (Inocarpus fagiferus Fosb.)
Author(s) -
Made Hany Anastasia,
Sri Rahayu Santi,
Manuntun Manurung
Publication year - 2016
Publication title -
jurnal kimia (journal of chemistry)/jurnal kimia (jurusan kimia, fakultas matematika dan ilmu pengetahuan alam universitas udayana)
Language(s) - English
Resource type - Journals
eISSN - 2599-2740
pISSN - 1907-9850
DOI - 10.24843/jchem.2016.v10.i01.p03
Subject(s) - chemistry , dpph , chloroform , antioxidant , flavonoid , chromatography , extraction (chemistry) , ethanol , organic chemistry
Gayam (Inocarpus fagiferus Fosb) is a tropical plant whose stem bark has been used as traditional medicine. This research aims to identify the flavonoides isolate from stem bark of gayam and identify the class of flavonoids compound as antioxidant. Extraction of 1000 g stem bark powder with 8500 mL of 96 % ethanol to yield 30 g concentrated ethanol extract. Partition of 15 g extract ethanol-water (7:3) with chloroform and n-buthanol to yield 1,5 g concentrated chloroform extract, 5,87 g concentrated n-buthanol extract and 5,63 g concentrated water extract. The flavonoids test using Wilstatter and Bate-Smith Matcalf reagent showed that n-buthanol and water extract contain flavonoids compounds. Antioxidant activity of flavonoids tested with DPPH method and lipid peroxide ammonium thiocyanate method showed that n-buthanol extract had antioxidant activity with IC50 of 24 ppm and n-buthanol extract has ability to inhibit the formation of lipid peroxide with inhibition value of 63,04 %. Separation of n-buthanol extract using thin layer chromatography preparative with chloroform as mobile phase and silica gel GF254 as stationary phase obtained four fractions. Flavonoids test showed that fraction F3 contained flavonoids compounds. FTIR analysis indicated that isolate had functional groups such as -OH , C=O, C-O alcohol, C=C aromatic, C-H aromatic and C-H aliphatic. UV-vis spectra showed 2 peaks at ? 310 nm and 261 nm which indicated flavonoids group of isolfavone with hydroxyl groups at C-5 and C-7. Antioxidant activity test with DPPH method showed that the isolate had antioxidant activity with IC50 of 1 ppm.