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This paper dealt with the preparation and characterization of self — assembled monolayersSAM-s of 1-hexadecanethiole and mercapto acetic acid on the silver nanostructure and subsequently the immobilization with amino acid L-Tryptophane. In order to achieve it, we used the electrodeposition of silver onto nanostructured surface of paraffin impregnated graphite electrode (PIGE). Subsequently, we assembled SAM by choosing the 1-hexadecanethiole and mercaptoacetic acid. These two kinds of SAM underwent the functionalization by L-Tryptophan. The observations of silver on PIGE surfaces were performed by scanning electron microscope (SEM). For surface analysis of the SAM functionalized by L-tryphophan, the TOF-SIMS technique was chosen. Finally, the fragmented ions of the immobilized-L-Tryptophan SAM were determined on the basis of suggested residues and three-dimensional structure. The residues show that the ability of L-Tryptophan to build homogeneous structure is better by mercaptoacetic acid SAM structure than by 1-hexadecanethiol. It was observed that L-Tryptophan built compact surface, which, due its chemical properties, can represent very interesting side regarding biocompatibility, homochirality and robustness in the area of life science.

TOF-SIMS surface analysis of L-Tryptophan self assembled monolayer