Open AccessAssociation Between ACE I/D Gene Polymorphism and Hypertension in Smokers
Author(s) -
Hateem Zafar Kayani,
Sana Riaz,
Nageen Hussain
Publication year - 2014
Publication title -
journal of advances in biotechnology
Language(s) - English
Resource type - Journals
ISSN - 2348-6201
DOI - 10.24297/jbt.v4i1.5006
Subject(s) - genotype , polymorphism (computer science) , medicine , blood pressure , genetics , essential hypertension , intron , gene , biology
The main aim is to study the association of ACE I/D polymorphism with healthy smokers. A total of 49 smokers and 49healthy controls were enrolled in this study. Intron 16 of the ACE gene was amplified by using nested PCR. The possiblebanding patterns were as follows; DD, 210 bp fragments; II, 264bp and 498 bp fragments; ID, 210 bp, 264 bp, and 498 bpfragments. The subjects comprised of males only. Among 49 subjects the frequency of ACE DD genotype was 75.5%(37/49), II 24.5% (12/49), while no ID genotype was found in the study. All controls showed DD genotype. The associationof ACE I/D polymorphism with different levels of blood pressure were found to be significant.