Open AccessEvaluation of Modified Oxidase, Cover-Glass Test, Antibiotic Sensitivity for Differentiation of Staphylococcus spp. from Other Related Genera
Author(s) -
Aws Ibrahim Sulaiman,
Basima A. Abdullah
Publication year - 2015
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2015.9.1.405
Subject(s) - nitrofurantoin , furazolidone , microbiology and biotechnology , antibiotic sensitivity , staphylococcus , staphylococcus aureus , biology , antibiotics , veterinary medicine , medicine , antibiotic resistance , bacteria , genetics
To comparison between modified oxidase, cover-glass, antibiotic sensitivity (Nitrofurantoin,Furazolidone) to evaluate which of them are most useful for differentiation Staphylococcus spp.from other related genera. A total of 115 clinical isolates out of them 38 isolates belongs toStaphylococcus spp. were collected from pus, urine and blood cultures of patients for both genderfrom period March to July 2012 at Al-Salam General Teaching Hospital in Mosul city. All isolateswere diagnosed and identified classically and using API test. The result indicated that Imipenemis highly effective against Staphylococcus spp. and all isolates were negative to modified oxidasetest except one isolate. All other isolates were given good growth under cover-glass test except one.67.5% of isolates were resistant to Nitrofurantoin and 32.5% were sensitive but 21.6% of isolateswere resistant to Furazolidone and 78.4% were sensitive. The above tests are the most tests indifferentiation between Staphylococcus spp. and other related Genera.