Open AccessExtraction and partial Purification of ipopolysaccaride (LPS) from E. coli O157:H7 ioslate
Author(s) -
Ashwak B. J. Al-Hashimi,
Essam F. Al-Jumaily,
Ami. Al-Thiwini,
Hitham A. Al-Omari
Publication year - 2009
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2009.3.1.34
Subject(s) - chromatography , extraction (chemistry) , chloroform , size exclusion chromatography , chemistry , nucleic acid , sepharose , petroleum ether , electrophoresis , lipopolysaccharide , phenol , ether , biochemistry , biology , enzyme , organic chemistry , endocrinology
Lipopolysaccharide (LPS) was partialy purified from E. coli O157:H7 local isolate by Sepharose – 4B gel filtration chromatography, after extraction by phenol-chloroform –petroleum ether mixture. The results show the presence of two peaks of proteins; while one peak of carbohydrates when tested at wave length 490nm. Nucleic acids were not found in the sample after partial purification. Electrophoresis pattern shows one large band with a molecular weight of 69000 daltons.