Open AccessDetection of bleomycin- like antitumor agent produced by Streptomyces spp. local isolates
Author(s) -
Asia J. Rweish,
Mohammed Ibrahim,
Rafid A. Abdul-karim,
Mohammed R. Abdul-majeed,
Zahra K. Jawad
Publication year - 2007
Publication title -
maǧallaẗ markaz buḥūṯ al-taqniyyaẗ al-aḥyāʾiyyaẗ
Language(s) - English
Resource type - Journals
eISSN - 2708-1370
pISSN - 1815-1140
DOI - 10.24126/jobrc.2007.1.1.9
Subject(s) - plasmacytoma , bleomycin , testis determining factor , fermentation , streptomyces , chromatography , chemistry , microbiology and biotechnology , biology , bacteria , biochemistry , multiple myeloma , gene , immunology , genetics , chemotherapy , y chromosome
Ethyl acetate extracts of fermentation cultures of five local Streptomyces isolates SRY-3, SRY-25, 5b, 10, NS-38 were identified as inhibitors of plasmacytoma cell line. Concentration at 0.016 µg/ml of ethyl acetate extract of (SRY-3,SRY-25,5b,10,NS-38) inhibited (46%,40%,21%,34%,34%) of mouse plasmacytoma cells respectively, where as 0.5 µg/ml inhibited (58%, 55%, 35%, 33%, 36%) of plasmacytoma cells .Quantities analysis of crude extracts by using HPLC on the basis of their retention times showed that the values were (4.3,4.4,4.86,4.83&4.84) min. for SRY-3, SRY-25, 5b, 10,NS-38 respectively, while the retention time of standard antitumor compounds was (4.26) min. This suggests that unknown compound of SRY-3 extract contain bleomycin-like compound. Addition of standard bleomycin to crude extract of SRY-3 increased milliabsorbans unit (M.A.U.) from 0.49 to 1.4. The concentration of bleomycin-like antitumor in fermentation broth of SRY-3 isolate was 3.441 µg/ml.The five Streptomyces isolates have moderate activity against gram-positive and gram-negative bacteria, while standard BLM have no activity.