Open AccessINVESTIGATION OF TROPHOBLAST IN TORCH INFECTION ON ABORTION MATERIAL IN THE FIRST TRIMESTER OF PREGNANCY
Author(s) -
A. V. Goshovskaya,
V. M. Goshovsky,
S. M. Yasnikovska
Publication year - 2019
Publication title -
klìnìčna anatomìâ ta operativna hìrurgìâ
Language(s) - English
Resource type - Journals
eISSN - 1993-5897
pISSN - 1727-0847
DOI - 10.24061/1727-0847.18.4.2019.9
Subject(s) - cytotrophoblast , trophoblast , chorionic villi , immunohistochemistry , andrology , pregnancy , gestation , syncytiotrophoblast , abortion , obstetrics , placenta , medicine , biology , gynecology , fetus , pathology , genetics
This study is a fragment of a series of immunohistochemical studies of trophoblast with TORCH infection, which are scheduled to be carried out at different gestational dates. This article deals with the results of trophoblast studies at the gestational age of 7-8 weeks. The study examined abortion material 7-8 weeks of gestation. The main group of the study consisted of 18 observations of TORCH infection, and the control group consisted of 17 observations of an aborted pregnancy without signs of an infectious process (abortion for social reasons). An immunohistochemical procedure was performed on metalloproteinases-2 with primary antibodies and polymer antigen imaging system using DAKO diaminobenzidine. The method of computer microdensitometry in a specialized computer program ImageJ evaluated the optical density of the color. According to the results of immunohistochemical studies using computer microdensitometry at the gestational age of 7-8 weeks, both with TORCH infection and without it an infectious process, strong expression of metalloproteinase-2 is observed in the invasive trophoblast, the smallest – in the syncytotrophoblast of the chorionic villi, and intermediate values are noted in chorionic villus cytotrophoblast and cell column cytotrophoblast. With TORCH infection the expression of metalloproteinase-2 decreases in all the four types of trophoblast (cytotrophoblast of chorionic villi; cytotrophoblast of cell columns; invasive cytotrophoblast in endometrial fragments), except for syncytotrophoblast of chorionic villi was found.