Open AccessVALIDATION OF A SIMPLES METHOD FOR SIMULTANEOUS DETERMINATION OF LIPOIC ACID AND RESVERATROL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Author(s) -
Irene Clemes KülkampGuerreiro,
Samuel Davies,
Sílvia S. Guterres,
Simone Jacobus Berlitz
Publication year - 2018
Publication title -
drug analytical research
Language(s) - English
Resource type - Journals
ISSN - 2527-2616
DOI - 10.22456/2527-2616.82578
Subject(s) - chromatography , high performance liquid chromatography , phosphoric acid , lipoic acid , chemistry , acetonitrile , resveratrol , antioxidant , organic chemistry , biochemistry
A high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed and validated for simultaneous determination of Resveratrol (RSV) and Lipoic Acid (LA). A C18 column was used with a mobile phase consisting of acetonitrile and 0.01M phosphoric acid (60:40). The detection wavelength was at 235 nm. The method was specific in the presence of pharmaceutical excipients widely used in solid dosage forms or lipid-core nanocapsules. The results demonstrated linearity between 5 and 50 µg/mL for RSV and 30 and 120 µg/mL for LA. The method presented precision and accuracy (RSD <5%). In addition, the developed method was considered robust. Therefore, the developed method can be applied successfully for simultaneous determination of RSV and LA in the proposed conditions, with a potential application to assay both drugs in several dosage forms.