Open AccessAnálise da microbiota de fissuras de dentes molares e pré-molares hígidos de humanos (Estudo quantitativo)
Author(s) -
Lucia Knewitz Boschetti,
Luiz Carlos Tovo,
José Oscar Schneider Santos,
Rui Vicente Oppermann
Publication year - 2021
Publication title -
revista da faculdade de odontologia de porto alegre
Language(s) - English
Resource type - Journals
eISSN - 2177-0018
pISSN - 0566-1854
DOI - 10.22456/2177-0018.118724
Subject(s) - veillonella , coccus (insect) , microbiology and biotechnology , agar , biology , bacteria , streptococcus , ecology , genetics
To analyze the groups of microrganisms present on fissures of caries free molars of 5 patients after 24h with no oral hygiene, it was collected the material deposited on its fissures after using erithrosin 0,25% as a disclosing agent. The material was cultivated in blood agar, for analysys of viable microrganisms, and in Veillonella agar, SL agar, TSA and McCarthy meidium, for the study respectevelly of Veillonella, Lactobacillus, iodophilic polysaccharides microrganisms and fusobacterium. The results of the viable microrganisms showed 58% of Gram positive coccus, 32% of Gram negative coccus, 10% of Gram positive rods. It was not found Gram negative rods.