Open AccessMOLECULAR-EPIDEMIOLOGICAL CHARACTERISTICS OF HIV1 VARIANTS CIRCULATING IN THE JEWISH AUTONOMOUS REGION TERRITORY
Author(s) -
В. О. Котова,
О. Е. Троценко,
Л. А. Балахонцева,
Е. А. Базыкина,
О. А. Янович,
Ю. В. Щиканов,
М. Н. Павлова,
Т. И. Шмакова
Publication year - 2019
Publication title -
vič-infekciâ i immunosupressii
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.176
H-Index - 4
eISSN - 2078-1792
pISSN - 2077-9828
DOI - 10.22328/2077-9828-2018-10-4-90-99
Subject(s) - sanger sequencing , reverse transcriptase , molecular epidemiology , drug resistance , virology , epidemiology , gene , human immunodeficiency virus (hiv) , genotype , coding region , genetics , biology , medicine , dna sequencing , polymerase chain reaction
Objective: to perform a molecular-epidemiological analysis of HIV-1 variants circulating in the Jewish autonomous region territory. Materials and methods. The research included 58 patients with HIV-infection. Amplified pol-gene fragments were used as a template to detect drug resistance mutations by Sanger sequencing (AmpliSens® HIV-Resist-Seq). Pol-gene is coding protease and a part of reverse transcriptase of HIV-1. Phylogenetic analysis was performed using MEGA version 6.0 program. Results: the research revealed an insignificant prevalence of HIV-1 СRF63_02A1 recombinant form. It was registered in 25 patients (44,6%). Sub-subtype A6 was identified in 23 HIV-positive people (41,1%). Subtype B was revealed in 6 cases (10,7%), subtype С — in two cases (3,6%). Primary drug resistance mutations were identified in 9 patients that were undergoing antiretroviral treatment. This dictates the necessity to change the treatment regimen in the specified patients. The prevalence index of drug resistance mutations in naïve patients equaled to 3,8%.