Open AccessExpressions of biomarkers in MCF7 Breast and Colon Cancer Cell Lines
Author(s) -
Poorima Bn Rao,
Farah Deeba
Publication year - 2020
Publication title -
journal of drug delivery and therapeutics
Language(s) - English
Resource type - Journals
ISSN - 2250-1177
DOI - 10.22270/jddt.v9i4-s.3993
Subject(s) - colorectal cancer , breast cancer , cell culture , cancer research , null cell , cancer , cell , histone , medicine , cancer cell lines , oncology , biology , cancer cell , genetics , gene
Cancer is one of the leading causes of death which accounts for 13% of total deaths, worldwide. Colorectal and Breast cancer fall under main categories according to World Health Organisation (WHO) cancer facts sheet 1. The need to understand the expression of clinical biomarkers in breast cancer and colon cancer is necessary for diagnosis and therapeutic response. In this article, the expressions of histone H1 and TP53 biomarkers were established for four different colon cancer cell lines and compared with the expressions of MCF7 cell line. The results show varied expression of Histone H1 along with TP53 markers among the cell lines. The results suggest that the linker Histone H1 has shown nuclear localization in HCT 116p53 wt (wild type) cancer cell line whereas it has shown cytoplasmic distribution in the respective null type cell line with intense expression. The result also suggests that a localized distribution in HRA19 cells and a diffused distribution in SNU-C2B cell line. These established results were compared with the expression of the biomarkers in MCF7 in order to get a better understanding.
Keywords: Tumour Proteins, p53, H1, MCF-7 cell, HCT116, HRA19.