Open AccessTHE TOXICITY OF MAULI BANANA (MUSA ACUMINATA) STEM WATER EXTRACT ON BONE MARROW MESENCHYMAL STEM CELL IN VITRO
Author(s) -
Amy Nindia,
Didit Aspriyanto,
Maharani Laillyza Apriasari,
Selviana Rizky
Publication year - 2019
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2019.v11s1.16762
Subject(s) - mesenchymal stem cell , stem cell , toxicity , in vitro , dose , chemistry , pharmacology , biology , biochemistry , microbiology and biotechnology , organic chemistry
Objective: Since mesenchymal stem cells (MSC) can differentiate into bone, cementum, and periodontal ligament, they can be used to treat aggressiveperiodontitis. The limited number of MSCs requires replenishment of growth factor in the cell culture process. Since growth factor is quite expensive,an alternative material is needed. Mauli banana stem has antioxidant and immunomodulatory properties. Methanol extract of Mauli banana stem isknown to be toxic toward MSCs; therefore, another solvent with a non-toxic effect is needed, such as a water solvent. We analyzed the toxicity of Maulibanana stem water extract on MSC in vitro.Methods: In this laboratory experimental (true experimental) study with a Post-test Only Control Group Design, MSC cultures were treated withMauli banana stem water extract at 10, 20, 40, 60, 80, and 100 mg/mL dosages. One group without any treatment served as a control group and onewas a media control group. Each group was incubated for 24 h and then was given 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidereagent and analyzed by an enzyme-linked immunosorbent assay (ELISA) reader.Results: One-way analysis of variance showed a significant difference.Conclusion: Mauli banana stem water extracts at 10, 20, 40, and 60 mg/mL were not toxic toward MSC in vitro, while dosages of 80 and 100 mg/mLdosage were toxic.