Open AccessCUMINUM CYMINUM EXTRACT REDUCES DENTAL PULP STEM CELL VIABILITY AT HIGHER CONCENTRATION
Author(s) -
Lipur Kurniawati,
Zaal Haq M,
Anggraini Margono,
Dewa Ayu Nyoman Putri Artiningsih
Publication year - 2019
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2019.v11s1.16015
Subject(s) - cuminum , dental pulp stem cells , viability assay , chemistry , pharmacology , traditional medicine , food science , biology , cell , medicine , in vitro , biochemistry , essential oil
Objective: Cuminum cyminum (cumin) extract has potential antibacterial and antifungal activities and is not toxic for mouse fibroblasts. However, toour knowledge, no research exists investigating the toxicity of cumin extract on dental pulp stem cells (DPSCs). Therefore, we compared the viabilityof DPSCs after treatment with different doses of the cumin extract (0.1, 0.4, 0.7, and 1.0 mg/mL) at 1 h, 24 h, and 4 days.Methods: DPSCs were gently evacuated from exfoliated third molars. Subsequently, cumin seeds were extracted by steam distillation toobtain 0.1, 0.4, 0.7, and 1.0 mg/mL concentrations. Then, the cell viability of DPSCs was analyzed using the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay by calculating the absorbance values using a microplate reader, with the optical density (OD) as the finalresult.Results: There were statistically significant differences in the viability of DPSCs (p<0.05) between 0.1 and 0.4, 0.7, and 1.0 mg/mL cumin extract, butthere was no difference (p≥0.05) in the viability of DPSCs at 1 h in each group.Conclusion: The cumin extract at all concentrations did not affect the viability of DPSCs at 1 h. However, the cumin extract at 0.4, 0.7, and 1.0 mg/mLdecreased the viability of DPSCs at 24 h and 4 days.