Open AccessTHE EFFECT OF CURCUMA XANTHORRHIZA - ETHANOL EXTRACT TO BIOFILM FORMATION OF STREPTOCOCCUS MUTANS AND AGGREGATIBACTER ACTINOMYCETEMCOMITANS (DENTAL BIOFILM RESEARCH: IN VITRO STUDY)
Author(s) -
Fidhianissa,
Hediyanti Joenoes,
Ariadna Adisattya Djais
Publication year - 2017
Publication title -
international journal of applied pharmaceutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.238
H-Index - 15
ISSN - 0975-7058
DOI - 10.22159/ijap.2017.v9s1.14_16
Subject(s) - streptococcus mutans , biofilm , aggregatibacter actinomycetemcomitans , chemistry , microbiology and biotechnology , curcuma , in vitro , crystal violet , ethanol , sucrose , food science , traditional medicine , periodontitis , bacteria , biology , medicine , dentistry , biochemistry , genetics , porphyromonas gingivalis
Objective: This in vitro study aimed to analyze the mass ratio of single- and dual-species Streptococcus mutans and Aggregatibacteractinomycetemcomitans biofilm after exposure to Curcuma xanthorrhiza ethanol extract (Xan).Methods: A bacterial suspension in brain heart infusion medium, enriched with 0.2% sucrose, was exposed to the Xan, incubated for 18 hrs, andanalyzed using a crystal violet assay.Results: This research concluded that the minimum inhibitory concentration of ethanol-temulawak extract against S. mutans was 5%, while theminimum bactericidal concentration was 15%.Conclusions: Xan prevented biofilm formation of single-species S. mutans and dual-species S. mutans and A. actinomycetemcomitans more effectivelythan it did single-species A. actinomycetemcomitans.