Open AccessANTIBACTERIAL, ANTIOXIDANT, CHEMICAL CONSTITUENTS, AND CYTOTOXICITY EVALUATION OF TERMINALIA ARJUNA (ROXB. EX DC.) WIGHT AND ARN
Author(s) -
Savita Khatri,
Neetu Phougat,
Mehak Dangi,
Anil Kumar Chhillar
Publication year - 2017
Publication title -
asian journal of pharmaceutical and clinical research
Language(s) - English
Resource type - Journals
eISSN - 2455-3891
pISSN - 0974-2441
DOI - 10.22159/ajpcr.2017.v10i3.16408
Subject(s) - dpph , chemistry , terminalia arjuna , antibacterial activity , antioxidant , cytotoxicity , traditional medicine , bacillus cereus , terminalia , chromatography , biochemistry , bacteria , biology , in vitro , medicine , genetics
Objective: The objective of this study is to evaluate the in vitro antibacterial and antioxidant prospective of Terminalia arjuna (leaves). The most activeextracts were examined for their chemical composition and cytotoxicity.Methods: The antibacterial activity of five different extracts were examined against 8 bacterial strains (5 Gram-positive and 3 Gram-negative) usingresazurin-based microtiter dilution assay (RMDA) and disk-diffusion assay. The antioxidant potential of five extracts was demonstrated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and superoxide radical scavenging assay. Chemical composition and cytotoxicity were assessed using gaschromatography-mass spectrometry (GC-MS) and hemolytic assay, respectively.Results: According to RMDA, the acetone extract (AE) exhibited highest antibacterial activity. The AE showed highest activity against Salmonellaenterica ser. typhi and Bacillus cereus with minimum inhibitory concentration, i.e., 195.31 μg/ml. In DPPH assay, AE showed the highest radicalscavenging activity with inhibition concentration50 23.09 μg/ml. In GC-MS analysis, the principal compound in AE was celidoniol (8.72 %). Accordingto the results of hemolytic assay, the AE showed non-toxic behavior upto 500 μg/ml.Conclusion: The present investigation represents T. arjuna as an incredible herb. The AE was found to possess promising antibacterial and antioxidantproperties.Keywords: Antibacterial, Antioxidant, Terminalia arjuna, Chemical composition, Cytotoxicity.