Open AccessШирокопольная флуоресцентная микроскопия в доклинических исследованиях биомедицинских материалов, скаффолдов и биомедицинских клеточных продуктов
Author(s) -
М.Н. Егорихина,
Д.Я. Алейник,
И.Н. Чарыкова,
Ю.П. Рубцова,
В. В. Юдин,
Alexander Morozov
Publication year - 2020
Publication title -
žurnal tehničeskoj fiziki
Language(s) - English
Resource type - Journals
eISSN - 1726-748X
pISSN - 0044-4642
DOI - 10.21883/jtf.2020.09.49695.15-20
Subject(s) - fluorescence microscope , adhesion , nanotechnology , cell adhesion , chemistry , cytotoxicity , fluorescence , materials science , biophysics , biology , in vitro , biochemistry , composite material , physics , optics
The results of studies representing the interaction of cells with various products provided for biomedical use are stated. It has been shown that wide-field fluorescence microscopy makes it possible to evaluate the cytotoxicity of the samples, the viability, morphology and proliferative activity of cells during the process of interaction, as well as the adhesion, migration, and distribution of cells, both on the surface of materials and in the structure of scaffolds and biomedical cell product. Methods of wide-field fluorescence microscopy make it possible to carry out intravital studies of the interaction of cells with materials without any additional processing and samples destruction.