Open AccessМодель генерации флуоресцентного сигнала интеркаляционного красителя в ходе полимеразной цепной реакции
Author(s) -
А. А. Федоров,
Д.Г. Сочивко,
Д. А. Варламов
Publication year - 2020
Publication title -
žurnal tehničeskoj fiziki
Language(s) - English
Resource type - Journals
eISSN - 1726-748X
pISSN - 0044-4642
DOI - 10.21883/jtf.2020.09.49693.422-19
Subject(s) - kinetics , chemistry , fluorescence , intercalation (chemistry) , chemical kinetics , polymerase chain reaction , chain reaction , product (mathematics) , photochemistry , physics , biochemistry , organic chemistry , mathematics , geometry , quantum mechanics , gene
Currently, a number of models of polymerase chain reaction have been proposed, claiming to be accurate quantitative estimates of the reaction results. However, all these models assume identity the kinetics of the reaction product and the used fluorescent reporter. In the present work a polymerase chain reaction model is proposed, product reaction kinetics of which are generated by intercalation dye. An analysis of the simulation results demonstrates noticeable differences between the kinetics of the reaction product and the fluorescent signal of the coupled intercalation dye.