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Oxidation and Phosphorylation By Mitochondria Isolated From Iron‐sufficient and Iron‐deficient Corn Roots
Author(s) -
Young Helen H.,
Wallace A.
Publication year - 1971
Publication title -
crop science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.76
H-Index - 147
eISSN - 1435-0653
pISSN - 0011-183X
DOI - 10.2135/cropsci1971.0011183x001100030012x
Subject(s) - mitochondrion , oxidative phosphorylation , isocitrate dehydrogenase , biochemistry , biology , malate dehydrogenase , enzyme , citrate synthase , dehydrogenase , phosphorylation , respiration , substrate (aquarium) , respiratory chain , succinate dehydrogenase , botany , ecology
Active mitochondria were successfully isolated from roots of Fe‐deficient and Fe‐sufficient corn ( Zea mays L.) plants. Citrate, DL‐isocitrate, α‐ketoglutarate, succinate, L‐malate, and NADH were oxidized by the mitochondria from either kind of corn plant. Fe‐deficient root mitochondria had decreased O 2 uptake and oxidative phosphorylation as compared to those of Fe‐sufficient root mitochondria. Furthermore, mitochondrla from Fe‐deficient corn roots had a low respiratory control ratio and the O 2 uptake had little response to the addition of ADP. Measurements of NADH oxidation by a spectrophotometric method showed that Fe‐deficient root mitochondria reduced the affinity between substrate and the nonheme Fe enzyme (NADH dehydrogenase).