Open AccessPENAMBAHAN L – ARGININ DALAM PENGENCER TRIS KUNING TELUR SETELAH EKUILIBRASI 2 JAM TERHADAP MOTILITAS DAN MEMBRAN PLASMA UTUH SPERMATOZOA DOMBA SAPUDI
Author(s) -
Hastini Suryaningsih,
Emy Koestanty Sabdoningrum,
Suherni Susilowati,
Trilas Sardjito,
Wurlina Wurlina,
Tita Damayanti
Publication year - 2020
Publication title -
ovozoa: journal of animal reproduction
Language(s) - English
Resource type - Journals
eISSN - 2722-967X
pISSN - 2302-6464
DOI - 10.20473/ovz.v7i2.2018.86-90
Subject(s) - arginine , diluent , semen , sperm , tris , yolk , andrology , motility , sperm motility , biology , chemistry , biochemistry , food science , medicine , amino acid , microbiology and biotechnology , nuclear chemistry
This research was aimed to determine the addition of L-arginine on motility and sperm plasma membrane of Sapudi sheep after two hour equilibration time in egg yolk tris diluent. This research used fresh samples of sheep Sapudi semen collected by artificial vagina, then were devided into 4 treatments and 6 replications. Analysis of the data using Analysis of Variance (ANOVA) then proceed to the Duncan Test to determine significant differences between treatments. The first treatment P0 was no L – Arginine added as control. P1 was treated with 0,004 M L – Arginine. P2 was treated with 0,005 M L – Arginine, P3 was treated with 0,006 M L – Arginine. Result of two hour equilibration time for the sperm motility showed: (P0) 46,67a 5,16, (P1) 49,16ab 5,84, (P2) 55,00bc 7,74, (P3) 58,33c 6,83. Result of two hour equilibration time for the sperm plasm membrane were: (P0) 39,33a 4,92, (P1) 43,33ab 4,22, (P2) 46,50 bc 3,44, (P3) 50,83 c 3,48. The addition of L-arginine with a concentration as much as 0.006 M, shows the highest result in sperm motility and sperm plasm membrane intact of Sapudi semen.