Open AccessPRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION
Author(s) -
Bagus Muhammad Ihsan,
Cecep Dani Sucipto,
Khayan Khayan
Publication year - 2021
Publication title -
journal of vocational health studies
Language(s) - English
Resource type - Journals
eISSN - 2580-717X
pISSN - 2580-7161
DOI - 10.20473/jvhs.v5.i1.2021.26-30
Subject(s) - primer (cosmetics) , microfilaria , polymerase chain reaction , high resolution melt , biology , melting curve analysis , real time polymerase chain reaction , filariasis , immunology , gene , genetics , helminths , chemistry , organic chemistry
Background: Filariasis patients can be a source of transmission if their blood still contains microfilariae. One of the Polymerase Chain Reaction (PCR) methods used is High Resolution Melting (HRM), using primary specificity testing. Purpose: To test the specificity of SLX primer. The samples used for this test were isolates of Salmonella., Klebsiella, Pseudomonas, negative and positive controls for Brugia malayi and Wuchereria bancrofti. Method: The design in this study is a quasi-experiment by testing the specificity of SLX primer using HRMbased real-time PCR based on the Cycle Threshold (CT) value observed through the amplification curve. Result: The real-time PCR results showed that no CT was released in the bacterial samples, and there was a CT value in the positive control. The results of this study indicate that specific SLX primer can be used in identifying microfilariae. Conclusion: SLX primer have a reasonable specificity because they cannot detect the existence of microorganisms in the samples other than microfilariae.