Open AccessTadalafil combined with remote ischemic preconditioning in the prevention of renal ischemia/reperfusion injury
Author(s) -
Ítalo Medeiros Azevedo,
Gabriela Vieira da Silva,
Aristela Sinara Varela Constantino de Medeiros,
Ariadne Sibelle Varela de Medeiros Dumaresq de Constantino Souza,
Rosana Karla Aires de Macêdo,
Aldo Cunha Medeiros
Publication year - 2018
Publication title -
journal of surgical and clinical research
Language(s) - English
Resource type - Journals
ISSN - 2179-7889
DOI - 10.20398/jscr.v9i1.15731
Subject(s) - tadalafil , medicine , nephrectomy , ischemia , urology , renal function , creatinine , ischemic preconditioning , renal ischemia , kidney , anesthesia , reperfusion injury , surgery , erectile dysfunction
PURPOSE: This study aimed to evaluate whether the combination of tadalafil treatment and remote ischemic preconditioning may provide protection against ischemia reperfusion injury (I/R) in kidneys of rats. METHODS: Twenty-four male Wistar rats weighing 280-300g were randomly divided into 3 groups: Group 1 - (control), only right nephrectomy was performed. No treatment with tadalafil. Group II - Left renal ischemia, right nephrectomy, without remote preconditioning, treatment with tadalafil (1.0 mg/kg, oral). Group III - Left renal ischemia, right nephrectomy, with remote preconditioning, treatment with tadalafil. RESULTS: 24 h after, urea and creatinine dosage and histopathology of the ischemic kidneys were performed. In the left kidney of control group rats, a higher degree of cellular necrosis was observed when compared to the tadalafil group, with and without remote preconditioning. On the preconditioning + tadalafil group rats, there was a significant reduction in histological parameters when compared to the Ischemia + tadalafil group rats (p<0.05). In group III, comparing to other groups, a significant improvement of the left kidney function was observed, through the lower values of the urea and creatinine dosages. CONCLUSION: This study demonstrated protective effects of remote preconditioning + tadalafil in an experimental model of renal I/R in the rat. The results demonstrated that a single dose of tadalafil prior to renal I/R attenuated the histopathological lesions, especially cellular necrosis, and renal function tests.