Open AccessThe Mixtures of 2.4-Dinitrophenylhidrazones of Inferior Carbonyl Compounds and their HPLC Separation with Gradient Binary Mixtures Phases
Author(s) -
Gheorghe Zgherea
Publication year - 2008
Publication title -
chemistry journal of moldova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.275
H-Index - 5
eISSN - 2345-1688
pISSN - 1857-1727
DOI - 10.19261/cjm.2008.03(1).02
Subject(s) - chemistry , boiling point , acetone , aldehyde , chromatography , high performance liquid chromatography , distillation , polarity (international relations) , organic chemistry , boiling , biochemistry , cell , catalysis
Mixtures of small quantities of carbonyl compounds are presents in foods, concerning sensorial qualities. The inferior carbonyl compounds (C2-C4, boiling point <100°C) – mono and dicarbonyl – can be identified and measured their concentrations, after a separation by distillation on the water bath. They are transferred in a strongly acid solution of 2.4-dinitrophenylhidrazine (2.4-DNPH), generating a mixture of insoluble 2.4-dinitrophenylhidrazones (2.4-DNPH-ones). The 2.4-DNPH-ones are organic compounds with weak polarity, solids, crystallized, yellows and water insoluble, soluble in organic solvents. The mixture of 2.4dinitrophenylhidrazones may be separated by liquid chromatography, using the reverse phase mechanism [1-3]. This paper contains experimental and theoretical considerations to the means of separation through liquid chromatography of two synthetically and a natural mixtures that contain 2.4-DNPH-ones provided by inferior carbonyl compounds; to obtain conclude results, in the synthetically mixtures was introduce and 2.4-DNPH-ones provided by carbonyl compounds having three (acetone and propanal) and four (isobutyl aldehyde) atoms of carbon.