Open AccessADAPTATION OF THE PROCEDURE OF CO-IMMOBILIZATION OF ENZYMES WITH DIFFERENT MODIFICATIONS OF ZEOLITES ON THE SURFACE OF CONDUCTOMETRIC TRANSDUCERS
Author(s) -
О. О. Солдаткін,
V. М. Arkhypova,
И. С. Кучеренко,
D. Yu. Kucherenko,
S. V. Dzyadevych
Publication year - 2021
Publication title -
sensorna elektronìka ì mìkrosistemnì tehnologìï
Language(s) - English
Resource type - Journals
eISSN - 2415-3508
pISSN - 1815-7459
DOI - 10.18524/1815-7459.2021.4.248177
Subject(s) - biosensor , urease , glucose oxidase , chemistry , zeolite , immobilized enzyme , conductometry , inorganic chemistry , chromatography , enzyme , catalysis , organic chemistry , biochemistry
A comparative study of different parameters of conductometric biosensors based on urease and glucose oxidase, co-immobilized with different types of zeolites. Urease immobilized on silicalite-2 was shown to have better performance than immobilized urease without zeolites. Conductometric biosensor with glucose oxidase co-immobilized with zeolite NH4+-Beta 25 had similar response values compared to immobilized enzyme without zeolite. Immobilization of zeolites NH4+-BEA 30 and H+-BEA 30 together with urease leads to an increase in the response of the biosensor, while the reproducibility of the signal remains unchanged. Biosensors with zeolites with a higher Si/Al ratio were characterized by increased signals. The use of zeolites modified with methylviologen and silver did not give a positive effect.