Open AccessDevelopment of an Indirect Fluorescent Antibody (IFA) Assay for the Detection of Leishmania RNA Virus 2 (LRV2) in Leishmania Parasites
Author(s) -
Homa Hajjaran,
Maryam Ebadizadeh,
Angila AtaeiPirkooh,
Mehdi Mohebali,
Katayoun Samimi-Rad,
Reza Saberi,
Saied Reza Naddaf
Publication year - 2022
Publication title -
iranian journal of parasitology./iranian journal of parasitology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.477
H-Index - 27
eISSN - 2008-238X
pISSN - 1735-7020
DOI - 10.18502/ijpa.v17i3.10625
Subject(s) - virology , biology , leishmania , antibody , serial dilution , leishmania major , virus , immunofluorescence , taqman , rna , direct fluorescent antibody , microbiology and biotechnology , polymerase chain reaction , gene , parasite hosting , immunology , genetics , medicine , alternative medicine , pathology , world wide web , computer science
Detection of Leishmania RNA virus (LRV) in Old World Leishmania species and their possible role in the disease prognosis requires sensitive and specific methods, preferably independent of the viral genome. We aimed to develop an indirect immunofluorescence antibody (IFA) assay to detect LRV in the Old World Leishmania parasites.