Open Access<FONT FACE=Symbol>b</font>-GALACTOSIDASE IMMOBILIZATION ON CONTROLLED PORE SILICA
Author(s) -
Henrique C. Trevisan,
E. P. Bergamo,
Jonas Contiero,
Ossamu Hojo,
Rubens Monti
Publication year - 1997
Publication title -
brazilian journal of chemical engineering/brazilian journal of chemical engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.313
H-Index - 52
eISSN - 1678-4383
pISSN - 0104-6632
DOI - 10.1590/s0104-66321997000400003
Subject(s) - chemistry , glutaraldehyde , lactose , hydrolysis , substrate (aquarium) , chromatography , chemical engineering , biochemistry , biology , engineering , ecology
The immobilization of β-galactosidase from Kluyveromyces fragilis on controlled pore silica was investigated. Immobilization was performed on amino silica activated with glutaraldehyde and the product was applied to the hydrolysis of lactose of whey. The behaviors of the soluble and immobilized enzyme were compared by using whey and a lactose solution as the substrate. With the aim of optimizing the method, parameters such as the amount of glutaraldehyde and the size of the particles were evaluated by comparing activities and stabilities on batch and continuously fluidized bed reactors