Premium
Cryopreservation of Channel Catfish Sperm: Storage in Cryoprotectants, Fertilization Trials, and Growth of Channel Catfish Produced with Cryopreserved Sperm
Author(s) -
Tiersch Terrence R.,
Goudie Cheryl A.,
Carmichael Gary J.
Publication year - 1994
Publication title -
transactions of the american fisheries society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.696
H-Index - 86
eISSN - 1548-8659
pISSN - 0002-8487
DOI - 10.1577/1548-8659(1994)123<0580:coccss>2.3.co;2
Subject(s) - cryoprotectant , catfish , cryopreservation , sperm , human fertilization , andrology , biology , ictalurus , sperm motility , gamete , botany , anatomy , fishery , embryo , medicine , fish <actinopterygii>
Abstract We developed methods for cryopreserving sperm of channel catfish Ictalurus punctatus and evaluated the use of cryopreserved sperm for reproduction. Five cryoprotectants were evaluated: Methanol, glycerol, dimethyl sulfoxide (DMSO), sucrose, and polyvinylpyrrolidone. We measured the motility of sperm that had been stored at 4°C in three concentrations of cryoprotectants (5%, 10%, 15%) dissolved in a modified Hankˈs balanced salt solution. All cryoprotectants reduced motility within 6 h; 5% methanol and 5% DMSO caused the smallest reduction. After sperm were frozen at –80°C and stored for 2 d at –196°C, motility was highest (5–10%) in samples cryopreserved with 5% and 10% solutions of methanol. Sperm cells cryopreserved in methanol solutions (5%, 10%, and 15%) were used to fertilize channel catfish eggs from three females. Fertilization ranged from 24% to 97%, and no difference in fertilization success was found between cryopreserved sperm and untreated sperm from the same males. Growth of channel catfish produced with cryopreserved sperm was not different from the growth of siblings produced with untreated sperm. Sperm cryopreservation offers utility as a routine method for gamete storage and genetic improvement of catfish.