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A Monomethine Cyanine Dye Cyan 40 for Two‐photon–excited Fluorescence Detection of Nucleic Acids and Their Visualization in Live Cells ¶
Author(s) -
Ohulchanskyy Tymish Y.,
Pudavar Haridas E.,
Yarmoluk Sergiy M.,
Yashchuk Valeriy M.,
Bergey Earl J.,
Prasad Paras N.
Publication year - 2003
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1562/0031-8655(2003)0770138amcdcf2.0.co2
Subject(s) - cyan , cyanine , excited state , fluorescence , two photon excitation microscopy , chemistry , photochemistry , fluorescence microscope , analytical chemistry (journal) , optics , physics , atomic physics , chromatography
ABSTRACT Monomethine cyanine dye 4‐((1‐methylbenzothiazolyliliden‐2)methyl)‐1,2,6‐trimethylpyridinium perchlorate (Cyan 40) was investigated as a two‐photon–excited fluorescence probe for nucleic acids (NA). Cyan 40 has been shown to demonstrate efficient two‐photon–excited fluorescence in the presence of NA in vitro in contrast to solutions without NA. Two‐photon confocal laser scanning microscopy (TPCLSM) and two‐photon laser scanning microspectrofluorometry were used to check the possibility of using Cyan 40 as two‐photon–excited fluorescence label for NA in living cells. Study of dye effect on viability of cells was also carried out. We ascertained that Cyan 40 is a cell‐permeant dye, manifesting efficient two‐photon–excited fluorescence when bound to NA in living cells, without any significant influence on viability of cells. TPCLSM images obtained from stained cells indicate preferential RNA staining by Cyan 40 compared with DNA.