Open AccessProduksi Dan Ekstraksi Inhibitor Alfa Glukosidase Dari Isolat Aktinomiset Jp-3
Author(s) -
Sri Pujiyanto,
Rejeki Siti Ferniah,
S Sunarno
Publication year - 2016
Publication title -
bioma : berkala ilmiah biologi/bioma
Language(s) - English
Resource type - Journals
eISSN - 2598-2370
pISSN - 1410-8801
DOI - 10.14710/bioma.17.2.123-129
Subject(s) - ethyl acetate , chemistry , chloroform , chromatography , extraction (chemistry) , substrate (aquarium) , fractionation , absorbance , methanol , enzyme , biochemistry , biology , organic chemistry , ecology
Alpha-glucosidase inhibitors are compounds that can prevent the digestion of complex carbohydrates into glucose, so potentially used as a diabetes drug. This study aims to examine the production and extraction of alpha-glucosidase inhibitor compound from Isolate Aktinomiset JP-3 from the sea. The supernatant obtained from the culture of the JP-3 isolate was extracted using various solvents to obtain the active compound. The solvents used were chloroform, methanol, and ethyl acetate. An assay of inhibitor activity of the α-glucosidase using p-nitrophenyl α-D-glucopyranoside substrate. The activity of the enzyme is measured based on the absorbance of p-nitrophenol produced from the breaking reaction of the substrate. The results showed that extraction of alpha-glucosidase inhibitor compound with ethyl acetate yielded extract with highest inhibitor activity. Keywords: alpha-glucosidase inhibitors, actinomycetes, diabetes, extraction, fractionation